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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is investigating a potential outbreak of a highly contagious respiratory virus. To rapidly screen a large number of nasopharyngeal swabs, the MOST appropriate initial diagnostic test would be:

A) Electron microscopy of the samples.
B) Whole-genome sequencing of viral RNA.
C) Rapid antigen detection assay.
D) Viral culture on a broad range of cell lines.

2. A clinical trial is evaluating the efficacy of a new antifungal agent against invasive aspergillosis. Serum galactomannan levels are being used as a biomarker to monitor the infection. A decrease in galactomannan levels during treatment would indicate:

A) Development of resistance to the antifungal agent.
B) Concurrent infection with a non-Aspergillus mold.
C) A false-positive result in the galactomannan assay.
D) Successful clearance or reduction of the Aspergillus burden.

3. A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:

A) Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
B) Increased production of para-aminobenzoic acid (PABA).
C) Enzymatic inactivation of trimethoprim and sulfamethoxazole.
D) Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.

4. A patient with a history of neutropenia following chemotherapy develops a severe pneumonia.
Bronchoalveolar lavage fluid reveals the presence of hyaline, septate hyphae with frequent dichotomous branching at acute angles. Culture yields a filamentous fungus that produces conidiophores with a vesicle bearing phialides that release chains of conidia. The MOST likely genus is:

A) Mucor
B) Aspergillus
C) Fusarium
D) Rhizopus

5. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?

A) Cysteine and pyridoxal
B) Both Factor X and Factor V
C) Factor V (NAD) only
D) Factor X (hemin) only

Solutions: